At first, import a ESR mix-file. 

Required command list. 
As an example separation of different charge states for 208Ni82+ is considered here:

(1)  SY           !calculations (control) of ion-optical parameters of the ring. Only do, if entire ring is used!
                  !The option "drübermalen.." (third button from right on the output window) has to be switched on 
                  !to plot different envelopes on one graph without clearing.      
(2)  KEMI,5,5     !emittance input, this determines beam size (mm mrad)
(3)  AKOP         !control of the beam parameters including the magnetic rigidity 
----- set stored ion species -----
(4)  KART,208,82  !input of the new beam parameters (a=208,z=82)
(5)  AKOP         !control of the beam parameters after the changes. The rigidity will change if a new beam is specified
(6)  KBET,-7.471  !Set the rigidity to the previous value by adjusting the beam energy in MeV/u: trial and error or you have to calculate the energy
(7)  SAVE         !save new settings
(8)  PREL         !initiate the change of the beam parameters leaving the ion-optical properties unchanged
(9)  SKIP         !back to saved settings again, necessary to refresh the output window
(10) LOE,PEN      !refresh the window
----- set product ion properties -----
(11) PREL         !must be executed again after the SKIP command
(12) KART,208,83  !input of new beam parameters (new charge state z=83)
(13) KART,208,84  !input of new beam parameters (new charge state z=84)
(14) KART,208,81  !input of new beam parameters (new charge state z=81)
(15) KART,208,80  !input of new beam parameters (new charge state z=80)
(16) HCOP,'testSeparation.eps',14  !save the output graph to *.eps-file
(17) SKIP
(18) LOE,PEN
(19) repeat (4-16) for other ions...
if you want to change the stored ion species again, you have to change from PREL mode to PNUL mode using the PNUL command, check current mode with MODE command